The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity). PTH_THET8 Peptidyl-tRNA hydrolase 183 TTHA1588 pth MFLVVGQGNPGERYARTRHNLGFMVLDRLGLSFRPRGEALVAEAEGGLFLKPLTYYNLTGRAVAPLARFYKIPPERILVVHDEMDLPLGRIRFKAGGSAAGNRGVLSIEEALGTRAFHRLRLGIGKPPDPSRGAEYVLSPFREEELPVVERVLEAAKEAVWCWVREGLPPCAGRFNGLDLSLG